The Government allocated $69.8million of new funding over four years to help eradicate bovine tuberculosis.
Primary Industries Minister Nathan Guy said the Government had made good progress in tackling the destructive disease.
"This new funding will help eradicate Tb from cattle and deer by 2026 and from Tb-infected wildlife in New Zealand by 2055.''
The amended Tb plan was a fundamental shift in approach from containing the disease to active eradication in livestock and wildlife, he said.
Since 2002, the number of infected cattle herds had reduced by 90%, and infected deer herds were down by 95%.
Tb had been cleared from wild animals in more than 1.2 million hectares of forest since 2011.
"This success means we can take a more targeted, scientific risk-based approach to both Tb testing for cattle and deer and wildlife control.''
The management agency for the plan was Ospri (Tb Free NZ), which carried out the work around the country.
The beef, dairy and deer sectors and the live cattle and deer animal-export sectors also contributed funding to the National Bovine Tuberculosis Pest Management sector.
Also in the Budget, Mr Guy announced $16million of new funding over four years to tackle wilding conifers.
The new funding would be used to control wilding conifers in the highest-priority areas.
They were likely to include the headwaters of the Southern Alps lakes and rivers, the Wakatipu basin, Molesworth Station and the North Island's central plateau.
Conservation Minister Maggie Barry said five groups tackling the wilding conifers had received $345,000 from the Community Conservation Partnerships Fund as part of the "War on Weeds''.
Wilding conifers covered more than 1.7 million hectares of land and were advancing at an estimated rate of 5% a year, she said.
At a glance
• $69.8million to help eradicate bovine tuberculosis
• $16million to tackle wilding conifers
• Community groups get funding to fight wilding conifers