The Southern Stars hold an imposing 8-2 lead in the multi-format series in England, after a 2-1 win in the three-game ODI component and a convincing 161-run victory in the one-off Test.
They can clinch the series by winning just one of the T20 games, and aim to seal it in game one in Chelmsford on Wednesday.
The only way England can retain the Ashes is by winning all three T20 matches which would pull them level at 8-8 and they would then keep the coveted trophy by virtue of being the defending holders.
It will be the first time the two nations have clashed in the game's shortest format since Australia scored a thumping six-wicket win in the 2014 World T20 final in Bangladesh.
However, England have a 12-8 all-time advantage over the Southern Stars in T20 cricket and an impressive 6-2 record on home decks.
Australia have lost their last five T20 matches to England in that country across tours in 2011 and 2013 and their last victory over the old enemy there was back in 2009.
However, Australia are currently riding a 16-game T20 winning streak against all comers since their last loss to New Zealand in April 2014, while England have won nine of their past 11 matches.
"I think the T20 series is going to be a really close one, the format suits both teams quite well," Lanning told AAP.
"We're sure that England will bounce back really hard from the Test match, so we know we're going to have to play really well to get the win we need."
England's changes to their vanquished Test team include calling up spinners Danielle Wyatt and Danielle Hazell, with the latter the world No 1 ranked women's T20 bowler.
"They have both been very effective in the past in T20 cricket so we're going to have to look at that and see how we combat them best," Lanning said.
Australia's two highest-ranked T20 bowlers Jess Jonassen (5th) and Erin Osborne (eighth) are also spinners.
Fast-scoring Lanning, who is the world No 1 ranked women's T20 batter just ahead of opposing captain Charlotte Edwards and another English women Sarah Taylor, is keen to get back among the runs again after scores off 3 and 0 in the Test.
"I didn't really contribute with the bat in the Test match, but thats the way it goes," Lanning said.
"Sometimes you've just got to take your medicine and move on, so I'm looking forward to the T20 series.
"It suits my game, I really enjoy playing T20 cricket."
