Scotland coach Vern Cotter kept faith with most of the players who performed so impressively in the Rugby World Cup when he named his Six Nations squad, but he included two uncapped props - Zander Fagerson and Rory Sutherland.
Glasgow tighthead Fagerson is 20 on Wednesday (local time) while 23-year-old Sutherland has earned a shot after a strong start to the season for Edinburgh.
Prop Moray Low and hooker Pat MacArthur were also included after missing out on the World Cup, where Scotland agonisingly lost to Australia in the quarter-finals.
There are 23 World Cup squad members retained, with six unavailable through injury or retirement.
Back into the frame come centres Alex Dunbar and Duncan Taylor, who missed the World Cup through injury, as does John flanker Barclay, a surprise omission last year.
Another notable absentee was Ryan Wilson, who was cited on Monday for grabbing an opponent's testicles in a European Champions Cup match against Northampton.
Cotter said that Wilson had been left out of the squad before the citing was announced.
"We're happy with the way he has been improving but we feel that the (other) players are playing better than he is at the moment," Cotter said on Tuesday.
Lock Tim Swinson, who has been cited for punching in the same match, was included in the squad.
"We've selected a mixture of experience with some exciting young players coming through, some of whom have experienced the Rugby World Cup and are eager to move forward, take the Six Nations head on and show that we can develop our game and be competitive," Cotter said in a statement.
"I think there should be confidence. The guys have worked hard and improved. There are positive signs that we can compete, and this championship will give us another opportunity to measure ourselves against very good opposition in a tough competition."
Scotland, who finished bottom of last year's Six Nations after losing every game, kick off the tournament at home to England on Feb. 6.
Forwards: Hooker Ross Ford (Edinburgh Rugby) - 94 caps Pat MacArthur (Glasgow Warriors) - 6 caps Stuart McInally (Edinburgh Rugby) - 2 caps
Front row Alasdair Dickinson (Edinburgh Rugby) - 52 caps Zander Fagerson (Glasgow Warriors) - uncapped Moray Low (Exeter Chiefs) - 29 caps Willem Nel (Edinburgh Rugby) - 8 caps Gordon Reid (Glasgow Warriors) - 15 caps Rory Sutherland (Edinburgh Rugby) - uncapped
Lock Jonny Gray (Glasgow Warriors) - 19 caps Richie Gray (Castres) - 51 caps Tim Swinson (Glasgow Warriors) - 17 caps Ben Toolis (Edinburgh Rugby) - 1 cap
Back row Adam Ashe (Glasgow Warriors) - 6 caps John Barclay (Scarlets) - 45 caps Blair Cowan (London Irish) - 15 caps David Denton (Bath) - 32 caps Chris Fusaro (Glasgow Warriors) - 4 caps John Hardie (Edinburgh Rugby) - 5 caps Josh Strauss (Glasgow Warriors) - 5 caps
Backs: Scrumhalf Sam Hidalgo-Clyne (Edinburgh Rugby) - 8 caps Greig Laidlaw CAPTAIN (Gloucester) - 46 caps
Stand-off Finn Russell (Glasgow Warriors) - 15 caps Duncan Weir (Glasgow Warriors) - 21 caps
Centre Mark Bennett (Glasgow Warriors) - 13 caps Alex Dunbar (Glasgow Warriors) - 14 caps Peter Horne (Glasgow Warriors) - 15 caps Matt Scott (Edinburgh Rugby) - 33 caps Duncan Taylor (Saracens) - 12 caps
Winger Sean Lamont (Glasgow Warriors) - 101 caps Sean Maitland (London Irish) - 20 caps Tommy Seymour (Glasgow Warriors) - 22 caps Tim Visser (Harlequins) - 23 caps
Fullback Stuart Hogg (Glasgow Warriors) - 38 caps Ruaridh Jackson (Wasps) - 27 caps
