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Police in Queenstown investigated the sighting of the British girl, but later issued a statement saying they were ''absolutely satisfied' it was not the girl who went missing in Portugal while holidaying with her parents, on May 3, 2007.
Detective Senior Sergeant Kallum Croudis, of Dunedin, said in a statement this week Scotland Yard had requested a DNA test.
''Police will be sending a DNA profile to British police investigating the disappearance of Madeleine McCann, to confirm the identity of a girl who has been mistaken for Madeleine by a member of the public.''
Results of the test, which was taken voluntarily and would provide a ''conclusive way to establish identity'', were expected within weeks, he said.
It was not the first time Southern district police have investigated an alleged sighting of the girl. A claimed sighting at The Warehouse in South Dunedin in 2007 was later confirmed by police as being of another child.
A Balclutha couple also reported seeing a man with a Madeleine lookalike, and other reports have come from Alexandra and Queenstown.
In the Dunedin case, police asked media outlets to not publish the grainy black and white security footage of the girl, after it was confirmed she was not Madeleine.