Mr Hughes is upset over the loss of his black and silver 27-speed Jamis Durango bike, which he had modified with a distinctive trailer attachment.
"I just want it back," Mr Hughes said.
The bike was taken from outside Ground Essence on George St between 7.30pm and 10pm.
"It had a lock on it, but someone took it away," Mr Hughes said.
The bike was locked through the wheels, but was not secured to anything.
"They would have had to pick the bike up and carry it, or drag it. One of the two."
Mr Hughes started his courier business earlier in the year after being made redundant from Fisher and Paykel in Mosgiel.
Mr Hughes' courier liability covers the packages he delivers, but not his personal property.
He will not be able to claim on his house and contents insurance cover either because the bike was not stolen from outside his house.
"I've been riding for 22 years. I don't hold grudges or anything - I just want the bike back."
- Rachel Taylor
