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The Dunedin City Council is restricting the area in a two-week trial.
Council transport group manager Richard Saunders said it was a chance to test the merits of any permanent change, as well as the reaction from the public and businesses and other interested parties.
It comes after a four-day trial in January last year, in which planter boxes were positioned to block vehicles from using the through-road.
At the time 80% of the 172 people surveyed supported the closure, a further 9% supported it with some reservations, and 11% of respondents were against the initiative.