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The apparent unprovoked assault happened about 2.45am on Saturday, outside Rodgers House, at the intersection of Princes and Dowling Sts.
Following the incident, the two alleged assailants were seen running down Dowling St towards Queens Gardens.
The victim was taken to hospital in a serious condition.
Police said yesterday they would like to hear from the men shown in the photos, or from anyone who could identify them.
-Dunedin police (03) (03) 471-4800; Crimestoppers 0800 555-111.