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Media personality Alison Mau will MC the sold-out event, themed Catalyst for Change, that targets women in business in Otago and Southland.
Chamber chief executive Ann Lockhart said there had been a strong demand for the event, which underlined the value of the forum for local business women.
''The conference is now oversubscribed after the 225 places were filled within just a few weeks.
''We have a waiting list and it highlights an increasing awareness of the professional quality of the programme we provide.
''Every year we set the bar higher and this year I am confident the range of speakers we have attracted will once again provide a significant point of difference.
''The mix of entrepreneurial skills and hands-on business experience amongst our talented line-up would equal any similar event in New Zealand.''
Mau has 30 years' experience as a broadcaster and journalist.
Speaking at the conference will be Celia Lashlie, the first woman in New Zealand to work in a custodial role in a men's prison; New Zealand footwear designer Kathryn Wilson; Queenstown motivational speaker and author Amanda Mortimer; 2010 Kiwibank New Zealander of the Year finalist Julie Chapman, co-founder and chief executive of KidsCan; Andrea Thompson, founder of New Zealand's leadership performance company Catapult; founder of Agri-Women's Development Trust and 2013 Next Business Woman of the Year Lindy Nelson; and style guru Lisa O'Neill, who will be making her third appearance at the conference.
• The conference will be held at Hilton Queenstown on May 16.