Queenstown will join Dunedin on the new Bee Card bus ticketing system today.
Bus fare collection will also resume and there are some timetable changes.
Adult fares remain at $2, while cash fares are $3, down from $5 per adult and $4 for those aged 5-18, The airport fare is now $4, down from $10 an adult.
Bee Cards do not have to be registered and passengers can top up on the bus, but registering a card allows top-ups to be made online.
Regional Transport Committee chairwoman Alexa Forbes said it would take slightly longer the first time a card was tagged on to a bus and processing top-ups could take up to 12 hours.
When credit was added on to registered cards, the balance would say "pending balance" until the card was used to tag on to a bus.
Approximately 77,000 cards had been activated for the Otago region and of those, over 23,200 cards were registered online.
In timetable changes, Route 1 will extend to Sunshine Bay; there will be additional peak trips on the Route 2 Arthurs Point and Arrowtown services; Shearers Rd has been added to Route 3; and Route 5 Lake Hayes Direct is now seven days per week.
There are new stops at Hanley’s Farm, Akarua Wines on Arrowtown-Lake Hayes Rd, and near Millbrook at the intersection of Malaghans Rd.
Regional council transport manager Garry Maloney said the changes reflected public feedback.