New coach Tony Brown has named a close to full strength side for the game at Brookvale Oval.
All Blacks vice captain Ben Smith is at fullback, having committed last week to New Zealand Rugby through to 2020, and is joined by test backs Waisake Naholo, Malakai Fekitoa, Lima Sopoaga and Aaron Smith.
The forward pack is bolstered by Liam Squire and Liam Coltman, while test flanker Elliot Dixon is on an 18-strong reserves bench for the Otago-based side.
Also on the bench is Fijian international winger Patrick Osborne.
A regular starter in recent years, Osborne makes way for Tevita Li from the Blues in a possible pointer towards Brown's thinking.
Hookers Ash Dixon (back) and Greg Pleasants-Tate (knee) weren't considered because of injury, along with five-eighth Marty Banks (knee).
The Highlanders lost their opening trial match 50-33 to the Crusaders two weeks ago.
They reached the quarterfinals at last week's Brisbane Global Tens, eliminated by Hurricanes.
Highlanders:
Ben Smith, Waisake Naholo, Malakai Fekitoa, Rob Thompson, Tevita Li, Lima Sopoaga, Aaron Smith, Luke Whitelock, James Lentjes, Liam Squire, Jackson Hemopo, Tom Franklin, Siate Tokolahi, Liam Coltman, Daniel Lienert-Brown. Reserves: Aki Seiuli, Craig Millar, Siosuia Halanukonuka, Guy Millar, Adrian Smith, Josh Dickson, Alex Ainley, Elliot Dixon, Gareth Evans, Dillon Hunt, Kayne Hammington, Josh Renton, Fletcher Smith, Sio Tomkinson, Teihorangi Walden, Matt Faddes, Pat Osborne, Jason Emery.
Reserves
Forwards: Aki Seiuli, Craig Millar, Siosuia Halanukonuka, Guy Millar, Adrian Smith, Josh
Dickson, Alex Ainley, Elliot Dixon, Gareth Evans, Dillon Hunt
Backs: Kayne Hammington, Josh Renton, Fletcher Smith, Sio Tomkinson, Teihorangi
Walden, Matt Faddes, Pat Osborne, Jason Emery
Injured: Ash Dixon (back) Marty Banks (knee) Greg Pleasants-Tate (Knee)