Three of the 18 passengers on the jet-boat, operated by Dart River Jet Safaris, received minor injuries, two apparently involving shoulders, and one a knee, when the boat overturned just north of the Dart River bridge, about 3.45pm.
Dart River Jet Safaris is owned by Ngai Tahu Tourism.
A St John spokeswoman said two people with minor injuries were taken by ambulance to the Lakes District Hospital, at Frankton.
The latest national accident investigation in Central Otago - although involving only minor injuries - follows several recent fatalities from boat accidents in the area, and what Queenstown Lakes District Council chief executive Duncan Field recently termed "the worst accident record on our waterways in this district for 25 years".
David Kennedy, southern regional general manager for Ngai Tahu Tourism, said yesterday the company was taking the Dart River incident seriously and everything possible was being done to provide for the passengers.
Emergency services had been called immediately to assess and treat the injured passengers.
The tourism organisation had also arranged for all the passengers, including those injured, to be taken to Queenstown for any assessment and treatment necessary.
"At this early stage, we do not have any information about what caused the accident. We have begun an investigation and have reported it to Queenstown Lakes district harbourmaster Marty Black."
Dart River Jet Safaris has operated the Dart River trip since 1988.
Maritime New Zealand, whose responsibilities include maritime safety, said yesterday that some of its investigators would be travelling to the scene yesterday or early today.
Staff from the Transport Accident Investigation Commission would also travel to the area.
A Chinese national, Yan Wang, died after being trapped under a Kawarau Jet boat which was believed to have flipped on a sandbar on the Kawarau River in late September last year.
An English-born doctor, Paul Joseph Woods (29), who was a postgraduate medical science student at the University of Otago, died when the jet-boat in which he was travelling hit a gravel bank and flipped on the upper stretches of the Matukituki River in December.
Two men, Anton Oskar Woitasek (34), project manager, of Lake Hayes, and Laurence Brett Singleton (51), contractor, of Queenstown, died when the private jet-boat in which they were travelling was in collision with a jet ski on a bend of the Kawarau River, near Frankton, in early January.
