Enterprise Dunedin finance and operations manager Suzanne Jenkins said the lines were markers designed to help cruise ship visitors navigate the centre city on foot.
A green line would run the length of the shuttle bus stop outside Toitū Otago Settlers Museum, where a pop-up i-Site would be run on large cruise ship days.
A blue line ran from the museum to the front of the Dunedin Railway Station and the start of lower Stuart St.
A broken blue line ran from the Fryatt St wharf to the Thomas Burns St crossing, near the pedestrian overbridge beside the railway station.
Ms Jenkins said the painted lines were permanent but could be removed if they were no longer needed.
The cost of the markings was $1400.
"They were added following feedback from both visitors and tour operators about the need for better wayfinding like that found in other centres, including Picton, Wellington and Tauranga."
Dunedin City Council staff, volunteers and shuttle bus drivers would explain the new markings when greeting cruise ship passengers at the Port Chalmers terminal and when they disembarked from buses.
QR codes on shuttles would also direct passengers to more information.
