Glassford (20) won the bronze medal in the mens' 200m individual medley in a time of 2min 07.82sec. It was a personal best time by two seconds and was worth 771 Fina points.
He is the only Otago swimmer to stand on the podium in individual events at the championships and has won a silver and two bronze medals.
He finished behind United Club swimmers Steven Kent (2min 05.27sec) and Brett Newall (2min 06.34sec).
Glassford was slow out of the blocks and trailed the leading swimmers by four seconds at halfway.
But he came home strongly to close the gap on the leaders. His 50m splits were 28.60sec, 34.64sec, 34.66sec and 29.92sec.
In the men's 400m freestyle club relay, the Waves team of Rhys Applegarth, Troy Blavert, Braden Doyle and Clive Cox finished fourth in 3min 40.49sec.
Melissa Ingram exorcised her demons to qualify for the Commonwealth Games last night.
Ingram, a Beijing Olympian who missed out on the world championships last year, produced a strong performance to win the women's 200m backstroke under the qualifying time.
Three competitors ducked under the qualifying marks on the penultimate night of finals. Ingram was joined by fellow North Shore swimmer Daniel Bell and Southland's Natalie Wiegersma, who both qualified in their second events. That raised the total of Games qualifying performances to nine, from seven swimmers.
Ingram went through the halfway point of the 200m backstroke in 1min 03.88sec before two 33sec laps to clock 2min 10.73sec, nearly 1sec inside the qualifying mark.
"I definitely felt a few demons, walking along the pool to the blocks tonight. It was maybe not quite as fast on the back half as I would have liked, but it is definitely my fastest swim in a fabric suit," Ingram said.
Wiegersma round off a brilliant championship with her second qualifying time in the gruelling 400m individual medley.
She cleared out from the field to win in 4min 41.15sec, nearly 2sec under the qualifying mark.
She showed plenty of strength to power home in 31.40sec for the final 50m freestyle to win by 15sec from North Shore's Grace Francis.
