Greene (26) qualified for the event in Alberta in July when she won the New Zealand title last month. She dominated the women's race at the Whakarewarewa Forest, Rotorua, to beat last year's champion, Megan Diametri (Auckland), by two of the 16km laps in the 24-hour event.
She covered 304km.
Diametri finished seventh at the world championships in Canada last year.
Travel and accommodation costs for Greene and her one-person crew will be about $10,000 and she is seeking sponsors to help her get to the championships.
Greene, a sales specialist at R and R Sports, had contested a 24-hour race in Australia and this was her second attempt at the ultra-distance event.
She got an early break on Diametri by going out fast from the start and kept extending her lead throughout the race.
"The hardest part was in the dark hours between 3am and 4am," she said.
During the early part of the race Greene boosted her energy by consuming gels and sports drinks, but during the night she stopped for two minutes each lap to consume hot milo, hot custard, mashed potatoes and pumpkin soup.
Greene tried her hand at multisport when she was a pupil at Otago Girls High School and tried adventure racing after leaving school.
For the past three years she has focused on mountain biking and has built up an impressive record.
She represented New Zealand for the first time at the Oceania championships in Rotorua in 2006 and followed this up with a World Cup event in Canada.
Greene finished 63rd at her first short-course world championships in Rotorua the same year.
But the race was too short.
Greene enjoys the longer endurance events, and has proved it with some top performances in major New Zealand events over the past two years.
She won the New Zealand marathon (84km) title at Mount Peel last year and this sparked her confidence and she has won three major events this year.
Greene won the South Island Cup in the national mountain bike series, the national hill climb title in Nelson and the 24-hour race,She rides her mountain bike to work every day and does gym sessions to improve her core strength, using a swiss ball and doing sit-ups.
Her longest training ride in preparation for the 24-hour race was one seven-hour ride.
"I have no problems with the longer races," she said.
