Tourists outside the Dunedin Railway Station yesterday morning were greeted with a large banner emblazoned "You Cruise, We Lose", which hung from the station’s balcony for about 10 minutes, before protesters took it down.
The Climate Liberation Aotearoa group were protesting the environmental impact of the "giant floating hotels".
About 15 protesters remained on the forecourt - holding banners and handing out leaflets.
Climate Liberation Aotearoa spokeswoman Rosie Cruickshank, of Dunedin, said the group wanted to draw attention to the issue at a Dunedin landmark popular with tourists.
"It’s entirely a form of luxury emissions - no-one needs to go on a cruise."
She wanted to see cruise ship and aviation emissions included in emission reduction plans.
"We’ve already had ... some people from the cruise industry and cruise association call for that ... and we’re asking for other travel agents and cruise companies to ask for that."
Protesters were also present on the streets of Port Chalmers and two paddleboarders on the harbour displayed a banner as the cruise ship departed in the afternoon.
Outside the railway station yesterday, Diamond Princess passenger Wayne McInerney, of South Australia, said the protest was "crap".
While he believed in climate change, he thought the protesters were focused on the wrong industry.
"It’s a bit silly - there’s worse things. Aeroplanes and rockets do more damage."
Diamond Princess passengers have already been met by protesters from Climate Liberation Aotearoa in Auckland, Wellington and Christchurch over the past week.
Port Otago commercial manager Craig Usher said while there was a precautionary police presence in Port Chalmers, there were no issues.
Protesters aside, the first day of the season had gone "extremely smoothly", he said.
"The collaboration between [NZ Transport Agency Waka Kotahi] and Downer has been excellent," Mr Usher said.
"It’s gone off very well."