Helicopters Otago managing director Graeme Gale said the actions of his pilot should serve as a warning.
"You want to fire lasers at my helicopter — if I get you, you're going to be dealt with appropriately through the court system.
"I won't show leniency for anyone."
Mr Gale said one of their rescue helicopters was "lasered" multiple times from a car as it was flying over the Palmerston area early yesterday morning.
Two senior paramedics on board were hit in the eyes by the laser and would both seek medical attention to ensure their eyes were not damaged, he said.
After the pilot contacted him, Mr Gale said he instructed them to follow the vehicle and would "100%" do it again.
"If we're in a position that we can locate and have them arrested and dealt with, I'll do it every time I can."
Police confirmed they received reports of a laser being shone into a helicopter from a vehicle about 1.40am yesterday.
Sergeant Matt Lee, of Dunedin, said the vehicle was stopped in Coast Rd, north of Warrington, and three occupants were inside.
"No laser pointer was located in the search of the vehicle."
Inquiries were ongoing.
Mr Gale said he believed the laser pointer was disposed of.
He took instances like this "very seriously".
"We stuck with the vehicle to actually get the police to apprehend the people that were actually in the vehicle.
"We will prosecute them — make no bones about it."
Aiming a laser pointer at an aircraft was illegal and it was "very disappointing" that it had happened again, Mr Gale said.
"This is continuing to happen on a reasonably regular basis of people lasering, not just us, but aircraft and that in general.
"They're playing a serious game and potentially they could take an aircraft out of the air."
