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Cruise Doubtful Sound with Real Journeys, at No 6, was the highest-ranking activity by a Queenstown-based operator in the home-grown chart announced this week, which was formed by 283,000 public votes split over 1600 attractions.
Hanmer Springs Thermal Pools and Spa took the top spot.
The 150km Otago Central Rail Trail was the highest-rated Otago "must-do" at No 5, followed by Taieri Gorge Railway (59), Arrowtown (63), Skyline Queenstown (91), TSS Earnslaw and Walter Peak High Country Farm Excursions (96) and Mt Difficulty Wines (100).
Fiordland attractions Cruise Doubtful Sound (6), Mitre Peak and Milford Sound (25), Fiordland National Park (30) and the 54km Milford Track (32) featured in the top 50.
Tourism Industry Association (TIA) chief executive Martin Snedden said the new 101 Must-Do's for Kiwis list highlighted the huge range of activities and experiences New Zealanders could enjoy in their own backyard.
"I encourage every New Zealander to get out over summer and visit a new destination or try an activity they have never experienced," Mr Sneddon said.
"The 101 Must-Do's list offers dozens of ideas and TIA's 1500 plus members look forward to giving visitors a fantastic time.
Domestic tourism adds $13.8 billion a year to New Zealand's tourism industry, with New Zealanders travelling in their own country contributing $38 million in economic activity every day of the year.