Smith had been rested after suffering concussion in the Super Rugby tournament's first round loss to the Chiefs last month.
He will start at fullback and be joined by namesakes Aaron Smith and Otago first five-eighth Fletcher Smith, who makes his first start at this level.
Young Otago flanker Dillon Hunt will also makes his starting debut for the Highlanders on Saturday night.
The Hurricanes have replaced the injured Nehe Milner-Skudder with youngster Jordie Barrett.
The match kicks off at 7.35pm.
Highlanders: Ben Smith (captain), Richard Buckman, Malakai Fekitoa, Rob Thompson, Tevita Li, Fletcher Smith, Aaron Smith, Luke Whitelock, Dillon Hunt, Elliot Dixon, Joe Wheeler, Tom Franklin, Siate Tokolahi, Liam Coltman, Daniel Lienert-Brown.
Reserves: Sekonaia Pole, Aki Seiuli, Guy Millar, Jackson Hemopo, Gareth Evans, Kayne Hammington, Marty Banks, Matt Faddes
Hurricanes: Jordie Barrett, Vince Aso, Matt Proctor, Ngani Laumape, Julian Savea, Beauden Barrett, TJ Perenara, Ardie Savea, Callum Gibbins, Brad Shields, Michael Fatialofa, Mark Abbott, Jeffery To’omaga-Allen, Dane Coles (captain), Chris Eves.
Reserves: Ricky Riccitelli, Ben May, Mike Kainga, Sam Lousi, Reed Prinsep, Te Toiroa Tahuriorangi, Otere Black, Wes Goosen.