The locks to Dr Tim Gardner's Mackenzie Health Centre. which is a satellite clinic of the Kurow Medical Centre, were jammed with wood on November 26.
Security cameras caught a man apparently tampering with the locks.
And while the doors were able to be opened the next morning, Dr Gardner said the attempted ''sabotage'' of the health centre could have put patients at risk.
''That kind of sabotage is just ludicrous, with intent,'' he said. ''There's no place for that kind of behaviour, that kind of treatment, especially when we're providing a service for people who wish us to be looking after them. To potentially put them at risk, this is as much against patients as it is against [us].''
Last year, Dr Gardner's contract was terminated with the Twizel Medical Centre following a dispute and he left after about seven years as a GP in the area.
At the time, Mackenzie Mayor Graham Smith said ''Dr Tim'' was well thought of in the community and ''his sudden dismissal angered a lot of people''.
The Otago Daily Times reported early this year that at a raucous High Country Medical Trust annual meeting, attended by more than 300 people, Dr Gardner failed in a bid to be elected to the board.
The Twizel Medical Centre continued to operate without Dr Gardner and he and wife Juliet Gardner bought the Kurow Medical Centre in September and shortly afterwards established the Mackenzie Health Centre in Twizel, which opened in late October.
Dr Gardner said the medical centres now had 450 patients on their books.
When he opened in Twizel, he thought the business was ''potentially at risk here'' so a security camera was installed.
''We happened to catch the perpetrator dead to rights outside the door, shoving things in the lock, wiggling them around, embedding them, wiggling them again, standing back, reading the blurb sheet on the front of the window, and then wiggling it again and then wandering off in a certain direction.''
A police spokeswoman said police were aware of the security camera footage and the allegations and were making inquiries.
On Friday, police said no arrests had been made.
