Services are continuing through the nearby Highgate Presbyterian Church, but the Roslyn church complex was recently sold to a Korean religious group, the World Missionary Society Church of God, for $370,000.
The church had become surplus after the merger of the Maori Hill and Roslyn parishes in 2005, to become the Highgate Presbyterian Church.
The Rev Geoffrey Skilton, one of the parish ministers participating in yesterday's 1.30pm service, said some people had been "disappointed" with past decisions.
The parish was nevertheless moving on and would not become "captive to the past", he said.
Several long-serving parishioners said they were sad to be no longer worshipping at a church they had been involved with for, in some instances, many decades, but were also responding positively.
Shirley McChesney, in her mid-80s, said it was "sad in a way" that a church she had been involved with for more than 60 years was closing.
But the memories of people who had been good to her and supported her would always remain, she added.
Betty Elliotte said she also felt mixed emotions.
"I have very happy memories of my days here," Mrs Elliotte said.
Moderator of the Presbyterian Church's Southern Presbytery the Rev Richard Dawson, who played a leading role in the closure ceremony, said there would always be "mixed feelings" over the closure of a church with "such a history".
Some older churches were not readily adaptable for multiple uses, and it was important to focus on the overall needs of parishioners, he said.
Also participating in yesterday's service were parish clerk Netta Noone and parish ministers the Rev Barry Kelk and the Rev Jono Ryan. The Rev Peter Wishart was the communion celebrant.
The 850sq m church, designed by architect J. Louis Salmond, was built in 1904.
