Poplar Lodge and landowners Adin May and Jackie Sly lodged two submissions opposing the resource consent application by the Arrowtown Trust to relocate the church and use it for small-scale commercial activities, such as an artists' gallery or office.
The church was intended to be part of the public heritage precinct being developed by the trust.
The public opening of the first stage, involving the restoration and modern reuse of the three miners' cottages, was expected in the autumn.
However, the Merioneth St lodge owners submitted the church did not have a historical connection to the 19th-century cottages.
Moving the church near Adams Cottage would be "inappropriate" because of its size and the confines of the section.
Mr May and Ms Sly said they were concerned the trust's intended commercial opening times for the reused church, of between 7am and 8pm, and the proposed entrance to the church via Romans Lane would disturb their guests.
They raised the issue of pedestrian safety on Romans Lane if couriers made several deliveries a day.
The trust had not identified the type of tenant who would lease the church, the frequency of deliveries or the effect of having more pedestrians in the area, the lodge owners said.
Mr May and Ms Sly requested conditions if the application was granted.
They included limiting the hours of church activity to between 9am and 6pm, defining its intended use, providing for rubbish and recycling and confirmation the trust had funds and was able to complete the project in a reasonable amount of time.
The owners said they supported the New Zealand Historic Places Trust suggestion the property at 6 Merioneth St be demolished and the church moved there.
Trust chairman David Clarke said yesterday Mr May and Ms Sly had some valid concerns, which he hoped would be allayed through the hearing process.
He declined to comment further until the hearing.
The other four submissions received by Lakes Environmental by the closing date yesterday were in favour of the move.
Arrowtown resident Rupert Iles said the proposed relocation site would "complement the area of historic buildings depicting a past era of our heritage".
A hearing was expected to be scheduled for mid-February, in Queenstown, Lakes Environmental planner Wendy Rolls said yesterday.
