At the Caledonian Ground on Saturday, Bixley hit the front around the top bend to win the annual mile handicap race from Peter Frew (Leith) and Katrina Andrew (Hill City).
The race was first held in 1935 when it was won by New Zealand cross-country champion Bill Pullar (Anglican) and it was won by John Walker's coach, Arch Jelley (Mornington), in the late 1940s.
"I'm a sports nut and will study all the names on the cup," Bixley said.
Bixley (38), a research assistant at Invermay, adds his own lustre to the cup because he is the only New Zealand 24-hour champion to have won the shorter track event.
Bixley covered 201km when he won the New Zealand title in Auckland two years ago.
He covered more ground when he reached 221km when finishing 48th from 150 runners at the world championships in France last year.
He will have another crack at the world championship when it is held in Wales in September.
Bixley almost won the Ness Cup two years ago but was pipped on the post by Kirsty Morris (Leith).
Bixley, running off a handicap of 50sec, looked a possible winner halfway round the third of the four laps.
He was lying fourth at the bell and chased Frew, who passed frontrunner Maria Sleeman (Caversham) with 300m left, down the back straight and took the lead 150m from home.
He won by nearly 3sec from Frew who held out a late challenge by Andrew.
Scratch man Lyndon Brown (Caversham) ran the fastest time of 4min 40.66sec while Kirsty Morris had the best women's time of 5min 11.16sec.
Daniel Balchin (Caversham) sprinted home from 250m to win the men's 800m in a personal-best 1min 55.03sec from Ben Jowsey (Hill City) 1min 57.70sec.
Balchin (20) passed through the 200m in 28sec and was timed at 57sec at the bell.
His last 400m was 58sec.
Former Otago runner Kellie Palmer (Canterbury University), a teacher at Timaru Girls' High School, easily won the women's event in 2min 11.62sec.
New Zealand champion Marshall Hall (Taieri) overcame a rain-soaked circle to win the men's discus with a throw of 49.32m.
This was a remarkable throw in a slippery circle that prevented him from turning with confidence.
Dean Rusbatch (Taieri) beat the rain when he threw the hammer a personal-best 51.44m to win the men's aged 15 and 16 event.
He is ranked second in the country.
Michael Scholten (Taieri) broke his own New Zealand masters aged 30 to 34 weight pentathlon record by 42 points when he finished third in the Otago championships with 1917 points.
The event was won by veteran Brian Senior (Ashburton) with 3222 points from Stephen Rusbatch (Leith) 2252.
Winifred Harding (Taieri) won the women's weight pentathlon with 2784 points from Fiona Harvey (Taieri) 2506 and Claire Giles (Caversham) 1914 points.
