The two stars of the 100m dash anchored their teams, the Americans in the inside lane and the South Africans in lane eight.
The Americans gave Singleton a slight advantage at the final change and with 40m to run he still held the advantage, but the world's most famous paralympian has not won his reputation lightly.
After winning the 400m this morning he said that the relay was the event that excited him the most, and the South African celebrated after his victory.
The South African team of Samkelo Mike Radebe, Arnu Fourie, Henry David Roos and Pistorius also set a championship record of 42.80, just 0.5secs outside the world record held by the Americans. Brazil was third (43.43secs).
Pistorius was again all class in the T44 400m. Back to a more familiar distance and despite the blustery conditions, he still set a new championship record of 48.37secs in defeating Germany's David Behre (51.40sec) and David Prince (52.35) of the United States.
American Tatyana McFadden could not have been more impressive in totally dominating the women's 400m T54 field to win by more than two seconds in 54.88secs from Canadian Diane Roy and Switzerland's Manuela Schaer.
It was her fourth gold of the championships (200m, 400m, 800m and 1500m) to go with a bronze in the 100m.
The first world record of the day was in the women's discus F35/36 where Mariia Pomazan threw 28.73m (1073pts) to smash the old record by more than three metres. Silver went to Poland's Renata Chilewska (25.22m, 994 pts) with Katherine Proud foot (Australia) taking the bronze with a championship record for the F36 class of 24.52m 958pts.
The men's 400m T38 also produced a world best, thanks to the 49.33sec run from Tunisia's Mohamed Farhat Chida. In a tight finish he just headed Australia's Evan O'Hanlon (49.72secs) with former world record holder Tim Sullivan (Australia) finishing third in 53.42secs.
Iran's Iranian Se Hussein Livery (61.48m) set a world record in the men's javelin F13 but had to bow to China's Pinkie Zhu for the gold medal. Zhu (F12) threw 61.90m was enough to head the Iranian. Serbia's Miles Garlic (60.81) was third.
The afternoon produced two world records. Switzerland's Jeffrey Ige three 13.75m in the F20 shot put to defeat Efstratios Nikolaidis (Greece) 13.14m with Poland's Krzysztof Kaczmarek (12.72m in third.
Later, Algerian Nassima Saifi (40.99) set another world record when she surpassed her old mark in the F57/58 women's discus. Silver went to Stela Eneva (BUL) with a throw of 39.84 while bronze medal winner Nadia Medjmedj (Algeria) set a championship record for her F57 class with her 29.60m throw.
