The Harbour team, led by 14-year-old Lydia Ko, is keen to make it back-to-back titles after beating Waikato in the final on the Miramar course last year.
Ko is highly rated on the world amateur scene and will again play at No 1 in a team which has changed little from last year.
She finished fourth in the New Zealand Women's Open and has the honour of qualifying for the United States Amateur.
Harbour's toughest opposition will come from four other North Island teams.
They are: Auckland, which has won the title 19 times, last year's finalist Waikato, Wellington and the local Bay of Plenty team.
The Auckland No 1 is world-rated golfer Cecilia Cho, while another national representative Emily Perry takes top spot in the Waikato quintet.
Meanwhile, a weakened Otago team has the task of emulating the team's performance last year when it finished in the top half after bottom placings the previous four years.
Otago is without the services of three top players with Monica Tulisi returning to Christchurch with the aim of joining the professional ranks.
Lisa Henderson was an acquisition from Southland last year and boosted Otago's hopes but, along with Kathryn Fletcher, is unavailable this year.
St Hilda's schoolgirl Laura Hoskin will play at No 1 this year and will be tested by the best players in New Zealand.
Arrowtown's Jo Hicks-Beach is at No 2, with Shani Arona making up the top trio at No 3.
This year sees the return of Liz McRae at No 4 and a former No 1, Susan Greig, at No 5.
Otago starts against Canterbury this morning and meets Hawkes Bay this afternoon.
After a bye tomorrow morning, Otago comes up against Aorangi in the afternoon.
Otago will need to start well as the going gets a lot tougher on Thursday with matches against Auckland and Waikato.
In the final round on Friday, the team comes up against Southland but will have to be wary after being trumped in the Salisbury Cup clash earlier this year.