The All Black, who had only been on the field for about 10 minutes after being injected at halftime, went down wincing holding his shoulder after being cleaned out at a ruck on the 50-minute mark.
He was assisted from the field by medical staff and was seen heading away in an ambulance after the game in Queenstown this afternoon.
Highlanders head coach Jamie Joseph said he had not had a chance to see Holland after the game, but he "didn't look very well".
"It looks like it's a shoulder injury,'' Joseph told the Otago Daily Times after the game.
"He was going for the ball at the ruck and somebody hit him - legally by the looks of things.
"The concern is that if it's something serious, we get on to it quickly."
It will be a big blow for the Highlanders if Holland — who was named world rugby breakthrough player of the year in 2025 — is set for a spell on the sidelines.
The Highlanders open their Super Rugby season against the Crusaders in Dunedin next Friday.
— Allied Media
