Staff from Westpac and the Millennium Hotel, plus residents including a family and Destination Queenstown chief executive Tony Everitt, donned rubber gloves and put rubbish collected from Fernhill to Frankton beach into bin liners.
The Care for our Coast programme was run by the Sir Peter Blake Trust.
Westpac was a foundation partner of the trust.
Bank manager Adam Campbell said 20 bags of refuse had been collected and were destined for the tip.
"It's something we do every year and part of our commitment to the natural environment.
"I can't understand why there's so much rubbish when there are bins so close," Mr Campbell said.
"Cigarette butts near park benches; we'd love to see less of them next year," he said.