Doc will present to staff a new ''organisational structure'' this morning at consultation meetings before releasing it publicly this afternoon.
The department has already cut more than 265 jobs in the past four years, 96 positions going during last year's restructuring.
In Otago, Doc has 143 positions and, while it did not lose any of those in the last round of restructuring, many positions were changed and some were now working with a national rather than local focus.
Public Services Association national secretary Brenda Pilott said staff were understandably nervous as they knew the Government was continuing to slash Doc's budget.
''In saying that, people are concerned about what's going to be announced ... and what the impact will be in terms of job losses on the ground.''
Any job losses in Otago would add to 106 public sector jobs lost in the region last year, mostly because of centralisation of departments such as the IRD, immigration and housing, she said.
''Over the past four years, Otago has lost about 154 public sector jobs, and Southland about 92 jobs.''
Forest and Bird was concerned if frontline staff were laid off it would have serious effects on the wildlife and landscapes Doc was supposed to protect.
Advocacy manager Kevin Hackwell said, while volunteers were crucial, Doc had a unique role in protecting flora and fauna in remote places that were impossible for volunteers to access.
''There is no substitute for on-the-spot work by a paid and highly skilled staff member based in a remote but critical part of the country.''
Green Party conservation spokeswoman Eugenie Sage said volunteers could not do the work of skilled staff or make up for the funding shortfall as the department was hoping.
''Last year, revenues from commercial sponsorship was only $3.3 million.''
The job cuts and the resulting loss of expertise undermined the country's 100% Pure brand, she said.
