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Emergency services rushed to Man St, in Queenstown's CBD, at 2.35pm yesterday following the crash between a motorbike and a car at a roundabout.
A woman at the scene said she saw the motorbike "go straight under the car''.
"Everybody was screaming - it was crazy,'' she said.
"It was really scary.''
She said it appeared the man on the motorbike had tried to turn down a street off the roundabout at the same time as a car was turning.
The motorcyclist could be seen lying face down on the road at the scene, surrounded by emergency service personnel.
A St John spokesman said the man was taken to Lakes District Hospital with serious injuries.